Pvuii takara
WebApr 19, 2013 · P53CON fragment long 474 bp was prepared by PvuII (TAKARA) digestion of supercoiled plasmid pPGM1 (pBSK containing a p53CON sequence AGACATGCCTAGACATGCCT) as described in [19], second fragment long 2513 bp was used as nonspecific competitor. WebThe 3.5-kb plasmid pHIG5-3 (2 .mu.g) was dissolved in a total volume of 50 .mu.l of a buffer containing 20 mM Tris-HCl (pH 7.5), 10 mM MgCl.sub.2, 10 mM dithiothreitol and 50 mM NaCl (hereinafter referred to as "Y-50 buffer"), and 4 units of the restriction enzyme PvuII (Takara Shuzo; hereinafter, unless otherwise specified, all the enzymes ...
Pvuii takara
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WebDec 8, 2010 · PvuII (3180) BamHI (978) pIRESpuro3 MCS. 911. CGATATCTGC GGCCTAGCTA GCGCTTAAGG CCTGTTAACC GGTCGTACGT GCTATAGACG … Webreaction containing 10 U restriction endonuclease of PvuII (TakaRa Biotechnology Co. Ltd, Dalian, China) and 1 reaction ... which existed a recognition site of restric-tion enzyme …
WebApr 9, 2024 · Lumiprobe官网lumiprobe中国官网 Lumiprobe中国代理商lumiprobe荧光染料授权代理商 WebTaKaRa制限酵素 認識配列検索ツール「Takara Cut-Site Navigator」 制限酵素の使用に際して Universal Buffer・Basal Bufferによる制限酵素活性表示システム 制限酵素Genome …
WebRestriction digestion was performed at 37 °C for 18 h following the manufacturer’s protocol using PvuII restriction enzyme for ARMS2 A69S (Takara Bio, Japan) and Hsp92II for CFH Y402H (Promega). All amplified products were electrophoresed on 1.5% agarose gel containing FloroSafe DNA stain (1st Base Asia). WebAt the indicated time intervals (in minutes), chromosomal DNA was prepared, digested with PvuII endonuclease (TaKaRa Bio), and subjected to pulsed-field agarose gel …
WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA …
WebSequence Author: Clontech (TaKaRa) Open in SnapGene. Try SnapGene for Free. Download Plasmid Download SnapGene Viewer. Explore Over 2.7k Plasmids: … howellsideWeb4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM367 · Revised 3/23 www.promega.com 6. Additional Protocols for Selected Restriction Enzymes Promega scientists have tested a subset of restriction enzymes for compatibility with rapid … hide and seek offlineWebSep 16, 2024 · To obtain the 5′-flanking region of AeNPR1a, genomic DNA was digested with EcoRV, DraI, StuI, and PvuII (TaKaRa, Dalian, China). Then, the digested DNA were purified and ligated separately with Genome Walker adaptors using the BD Universal Genome Walker™ kit (Clontech, Mountain View, CA, USA) according to the … howell shooting supplyWebgested with restriction endonucleases (PvuII or XbaI; Takara, To-kyo, Japan), and electrophoresed in a 1.0% agarose gel. The poly-morphic PvuII and XbaI sites were … hide and seek overwatch codeWebSep 16, 2024 · To obtain the 5′-flanking region of AeNPR1a, genomic DNA was digested with EcoRV, DraI, StuI, and PvuII (TaKaRa, Dalian, China). Then, the digested DNA were purified and ligated separately with Genome Walker adaptors using the BD Universal Genome Walker™ kit (Clontech, Mountain View, CA, USA) according to the … hide and seek on the app storehowells hvacWebthe CoA on Takara Bio website. Relative Activity in Takara Bio's Universal Buffers : Universal Buffer L M H K T (+BSA) Relative Activity (%) (80) 100 40 <20 (40) ( ): Weak … howells house of fraser cardiff